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KMID : 0380020070220020109
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 2 p.109 ~ p.113
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Effect of Glycine Betaine on Follicle-Stimulating Hormone Production by Chinese Hamster Ovary Cells at Low Culture Temperature
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Yoon Sung-Kwan
Ahn Yong-Ho
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Abstract
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Suspension culture of recombinant Chinese hamster ovary (CHO) cells producing follicle-stimulating hormone was performed to investigate the effect of glycine betaine on cell growth and FSH production at low culture temperature. At 28°C, cell growth was suppressed, but cell viability remained high for a longer culture period. When the culture temperature was lowered from 37°C to 28°C, more than 14-fold increase in the maximum FSH titer was achieved. In batch culture at 28°C, the use of 15 mM glycine betaine (GB) to culture medium resulted in the enhancement of maximum cell density and FSH titer by 11% and 17%, respectively, compared to the culture without GB. In pseudo-perfusion culture at 28°C with the exchange of fresh medium containing 15 mM GB, a final FSH of 2,058 μg which is approximately 1.4-fold higher as compared to the culture without GB was obtained. This enhanced FSH production with 15 mM GB was not just because of enhanced specific FSH productivity (qFSH), but mainly because of the extended culture longevity. Taken together, this result demonstrates that the application of GB at low culture temperature is feasible to enhance the production of recombinant proteins in rCHO cells.
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KEYWORD
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Glycine betaine, CHO cells, Low culture temperature, follicle-stimulating hormone
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